Biolegend, 167902, Purified anti-mouse Siglec-G Antibody, 100μg

Siglec-G is a member of the CD33-related Siglec (sialic acid–binding, immunoglobulin-like lectin) family. Siglec-G is the orthologue of human Siglec-10. It is a 110-120 kD, 688 amino acid type I transmembrane protein and contains five extracellular immunoglobulin domains and an intracellular region with three conserved tyrosine-based motifs. It is an inhibitory receptor that is expressed in a B cell–restricted way, with particularly high expression in Pre-B and B1a cells. Upon stimulation with an antigen, Siglec-G is recruited to IgM, which is further phosphorylated inhibiting IgM-induced Ca2+ signaling and NFATc1 expression.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F - Verified IHC-P - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clones W20144D and SH1 can block the binding of each other.
RRID: AB_3716906 (BioLegend Cat. No. 167902)
Structure: Type I transmembrane protein that contains five extracellular immunoglobulin domains and an intracellular region with three conserved tyrosine-based motifs.
Distribution: B cells
Function: Inhibitis IgM-induced Ca2+ signaling and NFATc1 expression
Ligand/Receptor: Lectin
Gene ID: 243958
UniProt: View information about Siglec-G on UniProt.org
Clone: W20144D
Regulatory Status: RUO
Other Names: Siglec-G
Isotype: Rat IgG2b, κ