Biolegend, 168204, PE anti-mouse Ly-6C Antibody, 100μg

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6Cis reported to be an indicator of memory CD8 + T cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Ly6C-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Reference clone HK1.4 completely blocks the staining of clone S22006G
RRID: AB_3698945 (BioLegend Cat. No. 168203) AB_3698945 (BioLegend Cat. No. 168204)
Structure: 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution: Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.
Function: After cross linking Ly6C regulate endothelial adhesion of CD8 T cells through activation of LFA-1 at the expense of Ly6C down-regulation on the cell surface.
Interaction: Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Cell Type: Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Jutila MA, et al. 1988. Eur J Immunol. 18:1819. Cerwenka A, et al. 1998. J Immunol. 161:97.
Gene ID: 17067
UniProt: View information about Ly-6C on UniProt.org
Clone: S22006G
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus C
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.