Biolegend, 168403, PE anti-mouse CD146 Antibody, 25μg

CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is a cell adhesion molecule that plays a role in cell-cell and cell-extracellular matrix interactions and its expression on vascular endothelial basement membranes facilitates TH17 cell entry into tissues. CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1-positive cells, and neutrophils. In mice, CD146 is a marker of natural killer cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFNγ than CD146- NK cells upon stimulation with target cells or activating antibodies.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3717151 (BioLegend Cat. No. 168403) AB_3717151 (BioLegend Cat. No. 168404)
Structure: CD146 is a transmembrane glycoprotein and belongs to the Ig superfamily of cell adhesion molecules.
Distribution: CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1+ cells, and neutrophils.
Function: CD146 has been reported to be functionally relevant for endothelial cell adhesion, angiogenesis, and NK cell function.
Interaction: Endothelial cells, Mesenchymal Stem Cells, Neutrophils
Ligand/Receptor: Laminin-411
Cell Type: Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Despoix N, et al. 2008. Eur. J. Immunol. 38:2855. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746. Flanagan K, et al. 2012. PLOS One. 7:e40443.
Gene ID: 84004
UniProt: View information about CD146 on UniProt.org
Clone: 29-1709
Regulatory Status: RUO
Other Names: CD146, S-Endo 1 antigen, MUC18, MCAM, Mel-CAM, A32 antigen
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.