Biolegend, 168806, Alexa Fluor® 647 anti-mouse Granzyme B Antibody, 100μg

Granzyme B, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1, is a 32 kD serine protease. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Clones S23006A and GB11 partially block each other.
Structure: 32 kD serine protease
Distribution: Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells
Function: Induction of cell death and apoptosis
Ligand/Receptor: Mannose-6-phosphate receptor
Cell Type: Neutrophils, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. Griffiths GM. and S. Isaaz, et al. 1993. J. Cell Biol. 120:885. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. Wagner C, et al. 2008. Mol. Immunol. 45:1761.
Gene ID: 14939
UniProt: View information about Granzyme B on UniProt.org
Clone: S23006A
Regulatory Status: RUO
Other Names: Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
Isotype: Rat IgG1, λ