Biolegend, 200702, Purified anti-rat CD8b Antibody, 500μg

CD8b antigen is also known as T8, Lyt2, Ly-2, and CD8β. It is a member of the immunoglobulin superfamily expressed on most thymocytes, some mature T cell subsets, and macrophages. CD8b forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
500μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CD8+ Wistar rat splenic T cell hybridomas
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIP, WB, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, immunohistochemical staining1,2 of acetone-fixed frozen sections and Zinc-fixed paraffin-embeded sections, in vitro blocking of allogeneic responses1, Macrophage stimulation3,4 and in vivo depletion.
Application References(PubMed link indicates BioLegend citation): Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP IHC WB) Kuhnlein P, et al. 1994. J. Immunol. 153:979. (IHC) Hirji N, et al. 1997. J. Immunol. 158:1833. Hirji N, et al. 1998. J. Immunol. 160:6004.
Product Citations: Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
RRID: AB_313883 (BioLegend Cat. No. 200702)
Structure: Ig superfamily, CD8β chain
Distribution: Most thymocytes, subset of mature T cells (i.e. including most T suppressor/cytotoxic cells), macrophages
Function: Co-receptor for TCR
Ligand/Receptor: MHC class I molecule
Cell Type: Macrophages, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. 2. Kuhnlein P, et al. 1994. J. Immunol. 153:979. 3. Hirji N, et al. 1997. J. Immunol. 158:1833. 4. Hirji N, et al. 1998. J. Immunol. 160:6004.
Gene ID: 24931
UniProt: View information about CD8beta on UniProt.org
Clone: 341
Regulatory Status: RUO
Other Names: CD8, Lyt3
Isotype: Mouse IgG1, κ