Biolegend, 201003, PE anti-rat CD68 Recombinant Antibody, 25μg

CD68, also known as macrosialin, is a 110 kD integral membrane glycoprotein from the lysosomal-associated membrane protein (LAMP) family. It is predominantly expressed on the intracellular lysosomes of monocytes and macrophages, and is considered a pan-macrophage marker in the rat. CD68 may have functions relating to phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions.
25μg
Verified Reactivity: Rat
Reported Reactivity: Bovine
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone QA20A71 can completely block the binding of parental clone to target cells. Both Cyto-Fast™ Fix/Perm Buffer Set and True-Nuclear™ Transcription Factor Buffer Set work well. This clone does not react to human and mouse.
RRID: AB_2936606 (BioLegend Cat. No. 201003) AB_2936606 (BioLegend Cat. No. 201004)
Structure: Lysosome-associated membrane glycoprotein; single-pass type I membrane protein, 110 kD
Distribution: Macrophages, Langerhans cells, and at low levels on dendritic cells
Function: May involved in cell-cell interaction or cell-ligand interaction
Cell Type: Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References: Dijkstra CD, et al. 1985. Immunology. 54:589-99. Damoiseaux JG, et al. 1994. Immunology. 83:140-7.
Gene ID: 287435
UniProt: View information about CD68 on UniProt.org
Clone: QA20A71
Regulatory Status: RUO
Other Names: Macrosialin, GP110, LAMP4, Scavenger Receptor D1 (SR-D1)
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.