Biolegend, 201412, PE anti-rat CD3 Antibody, 100μg

CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: F344 rat spleen cells stimulated with PMA and calcium ionophore
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4,5 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2.
Application References(PubMed link indicates BioLegend citation): Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP) Nicholls MR, et al. 1993. Transplantation 55:459. (Block) Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF) Baba T, et al. 2006. Blood 107:2004. (IHC) Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P) Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed
Product Citations: Subas Satish HP, et al. 2022. MAbs. 14:2106621. PubMed Shahjin F, et al. 2023. Acta Biomater. 158:493. PubMed Johnson DC, et al. 2020. Cell Death Dis. 0.894444444. PubMed Longoni A, et al. 2020. Front Bioeng Biotechnol. 0.785416667. PubMed Huang Z, et al. 2021. Food Chem Toxicol. 152:112239. PubMed Córdoba-Moreno MO, et al. 2020. Sci Rep. 10:4799. PubMed
RRID: AB_2563272 (BioLegend Cat. No. 201411) AB_2563273 (BioLegend Cat. No. 201412)
Structure: Ig superfamily, approximately 20-25 kD
Distribution: Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC complex
Cell Type: NKT cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Tanaka T, et al. 1989 J. Immunol. 142:2791. 2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.
Gene ID: 2571030067831560925300
UniProt: View information about CD3 on UniProt.org
Clone: 1F4
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Mouse IgM, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.