Biolegend, 201517, APC/Cyanine7 anti-rat CD4 Antibody, 25μg

CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.
25μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat thymocyte membrane glycoproteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.
Application References(PubMed link indicates BioLegend citation): Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC) Shioji K, et al. 2001. Circulation Res. 89:540. (IHC) Mannie MD, et al. 1993. J. Immunol. 151:7293. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
Product Citations: Xie L, et al. 2014. J Immunol. 192:6009. PubMed Fehrenbach DJ, et al. 2020. Exp Physiol. 105:864. PubMed Abais-Battad JM, et al. 2021. Acta Physiol (Oxf). 232:e13662. PubMed Pai AV, et al. 2018. Physiology (Bethesda). 33:254. PubMed Alsheikh AJ, et al. 2020. Am J Physiol Renal Physiol. F982:318. PubMed Yanai H, et al. 2022. Elife. 11:. PubMed Li B, et al. 2020. Cell Mol Immunol. 1.386111111. PubMed Dasinger JH, et al. 2021. Pregnancy Hypertens. 24:126. PubMed Almolda B, et al. 2011. PLoS One. 6:e27473. PubMed Alsheikh AJ, et al. 2019. Am J Physiol Regul Integr Comp Physiol. 317:R182. PubMed Ruppert KA, et al. 2018. Sci Rep. 8:480. PubMed
RRID: AB_1186088 (BioLegend Cat. No. 201517) AB_1186084 (BioLegend Cat. No. 201518)
Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
Function: T cell co-receptor, T cell activation
Ligand/Receptor: MHC class II molecule
Cell Type: Macrophages, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609. 2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.
Gene ID: 2493824939
UniProt: View information about CD4 on UniProt.org
Clone: W3/25
Regulatory Status: RUO
Other Names: T4, L3T4, W3/25
Isotype: Mouse IgG1, κ