Biolegend, 201520, PerCP/Cyanine5.5 anti-rat CD4 Antibody, 100μg

CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat thymocyte membrane glycoproteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.
Application References(PubMed link indicates BioLegend citation): Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC) Shioji K, et al. 2001. Circulation Res. 89:540. (IHC) Mannie MD, et al. 1993. J. Immunol. 151:7293. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
Product Citations: Zhang Y, et al. 2024. Biology (Basel). :13. PubMed Giri T, et al. 2022. Heliyon. 8:e09407. PubMed
RRID: AB_2814097 (BioLegend Cat. No. 201519) AB_2814098 (BioLegend Cat. No. 201520)
Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
Function: T cell co-receptor, T cell activation
Ligand/Receptor: MHC class II molecule
Cell Type: Macrophages, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609. 2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.
Gene ID: 24932
UniProt: View information about CD4 on UniProt.org
Clone: W3/25
Regulatory Status: RUO
Other Names: T4, L3T4, W3/25
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.