Biolegend, 201902, Purified anti-rat CD11a Antibody, 100μg

CD11a is a 160-170 kD glycoprotein also known as α L integrin, or LFA-1 α chain. It is a member of the integrin family and forms a heterodimer with β 2 integrin (CD18). The LFA-1 (CD11a/CD18) is broadly expressed on leukocytes and mediates intercellular adhesion and cellular activation. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are the ligands of LFA-1.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: PHA-stimulated rat splenocytes and rat thymic lymphoma FTL-43
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedBlock, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The WT.1 antibody recognizes both activated and unactivated LFA-1 and inhibits the binding of LFA-1 to ICAM-1. The WT.1 antibody has been shown to inhibit the mixed lymphocyte reaction, leukocyte infiltration, and graft rejection. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of cellular adhesion3-6.
Application References(PubMed link indicates BioLegend citation): Andrews FJ, et al. 1997. Dig. Dis. Sci. 42:326. (IHC) Springer TA. 1994. Cell 76:301. (IHC) Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. (IP, Block) Devine L, et al. 1996. Immunol. 88:456. (Block) Ohta Y, et al. 1998. Transplantation 65:1094. (Block) Watanabe T, et al. 1997. Am. J. Pathol. 150:971. (Block)
Product Citations: Moran J, et al. 2023. Front Immunol. 1235675:14. PubMed
RRID: AB_2749874 (BioLegend Cat. No. 201902)
Structure: 160-170 kD glycoprotein
Distribution: Leukocytes including lymphocytes, monocytes/macrophages, and granulocytes.
Function: Mediates intercellular adhesion and cellular activation.
Interaction: CD18
Ligand/Receptor: CD54 (ICAM-1), CD102 (ICAM-2), CD50 (ICAM-3)
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. 2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:855.
Gene ID: 308995
UniProt: View information about CD11a on UniProt.org
Clone: WT.1
Regulatory Status: RUO
Other Names: LFA-1 α chain, αL Integrin, Lymphocyte function-associated antigen 1, LFA-1 α subunit, ITGAL, Ly-15, Ly-21
Isotype: Mouse IgG2a, κ