Biolegend, 202818, PerCP/Cyanine5.5 anti-rat CD43 Antibody, 100μg

CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and W3/13, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat thymocyte membrane glycoproteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections1 and paraffin-embedded sections4.
Application References(PubMed link indicates BioLegend citation): Barclay AN. 1981. Immunology 42:593. Brown WRA, et al. 1981. Nature 289:456. Forbes RC, et al. 1983. Transplantation 36:681. Jung S, et al. 1994. Immunology 83:545.
RRID: AB_2728226 (BioLegend Cat. No. 202818)
Structure: Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130kD
Distribution: Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.
Function: Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand/Receptor: CD54, Siglec-1
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238 Killeen A, et al. 1987. EMBO J. 6(13):4029. Thurman EC, em>et al. 1998. Intl. Immunol. 10:691. Onami TM, et al. 2002. J. Immunol. 168:6022. Tong J, et al. 2004. J. Exp. Med. 199:1277. Jones AT, et al. 1994. J. Immunol. 153:3426.
Gene ID: 20737
UniProt: View information about CD43 on UniProt.org
Clone: W3/13
Regulatory Status: RUO
Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, W3/13
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.