Biolegend, 205606, APC anti-rat CD161 Antibody, 100μg

CD161 is a 30 kD type II transmembrane C-type lectin, expressed as a homodimer. Rat NKR-P1 receptors are primarily expressed on NK cells, a subset of T cells, dendritic cells, and activated monocytes. There are three different types of NKR-P in rat, namely NKR-P1a, NKR-P1b, and NKR-P1c. NKR-P1a does not contain an ITIM structure and is an activating receptor, while NKR-P1b contains an ITIM and displays inhibitory function. LLT-1 (ligand lectin like transcript 1) is the ligand, while KLR (killer cell lectin like) functions as a receptor.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: LEW rat splenic NK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: 3.2.3 recognizes a common epitope of NKR-1P1a (CD161a) and NKR-P1b (CD161b).
Application References(PubMed link indicates BioLegend citation): Chambers W, et al. 1989. J. Exp. Med. 169:1373. (FC)
Product Citations: Noto FK, et al. 2020. PLoS One. 15:e0240169. PubMed Chen C, et al. 2018. J Neuroinflammation. 15:167. PubMed Hall JM, et al. 2021. Infect Immun. 89:e0030421. PubMed Hall JM, et al. 2021. Infect Immun. 89:e0034621. PubMed Ruppert KA, et al. 2018. Sci Rep. 8:480. PubMed
RRID: AB_11142680 (BioLegend Cat. No. 205606)
Structure: Type II transmembrane C-type lectin, 30 kD.
Distribution: NK cells, subset of T cells, dendritic cells, activated monocytes.
Function: NKR-P1a is a NK cell-activating receptor; NKR-P1b is a NK cell-inhibitory receptor.
Ligand/Receptor: LLT1-ligand lectin like transcript 1; KLR-killer cell lectin like receptor.
Cell Type: Dendritic cells, Monocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: CD Molecules
Antigen References: 1. Chambers WH, et al. 1989. J. Exp. Med. 169:1373. 2. Brink M, et al. 1990. J. Exp. Med. 171:197. 3. Li J, et al. 2003. Int. Immunol. 15:411.
Gene ID: 362443
UniProt: View information about CD161 on UniProt.org
Clone: 3.2.3
Regulatory Status: RUO
Other Names: NKR-P1, CD161a/CD161b, NKR-P1a/KLRB1a
Isotype: Mouse IgG1, κ