Biolegend, 205802, Purified anti-rat CD1d Antibody, 100μg

CD1d is a type I transmembrane protein and member of the MHC family that associates with β2-microglobulin on the cell surface. CD1d is expressed by antigen presenting cells (such as dendritic cells, monocytes, macrophages and B cells), thymocytes, and intestinal epithelial cells. CD1d presents glycolipids to Vα14 i TCR + i NKT cells.
100μg
Verified Reactivity: Rat, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: M12.4.1.C3 cells transduced with rat CD1d
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F, IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: blocking of CD1d-mediated antigen presentation to type I and II NKT cells1, immunoprecipitation1, Western Blotting1, and immunohistochemical staining of frozen tissue sections1. Clones WTH-1 and WTH-2 recognize different epitopes of CD1d.
Application References(PubMed link indicates BioLegend citation): Monzon-Casanova E, et al. 2010. PLoS ONE 5:e13089.
RRID: AB_11147755 (BioLegend Cat. No. 205802)
Structure: Member of the MHC family, type I transmembrane protein, associates with β2-microglobulin
Distribution: Dendritic cells, monocytes, macrophages, B cells, T cells, thymocytes, intestinal epithelial cells
Function: Presents glycolipid antigens to iNKT cells
Ligand/Receptor: Vα14i TCR
Cell Type: B cells, Dendritic cells, Epithelial cells, Macrophages, Monocytes, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, MHC Antigens, TCRs
Antigen References: 1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA. 107:10984. 2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663. 3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA. 107:1535. 4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.
Gene ID: 25109
UniProt: View information about CD1d on UniProt.org
Clone: WTH-1
Regulatory Status: RUO
Other Names: Antigen-presenting glycoprotein CD1d, Cd1d1
Isotype: Mouse IgG2a, κ