Biolegend, 206106, FITC anti-rat CD90 Antibody, 500μg

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, and stromal cells.
500μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: PC12 cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for relevant formats of this clone) include: immunoprecipitation and Western blotting. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 206104) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg)
Application References(PubMed link indicates BioLegend citation): Kuroiwa K, et al. 2012. Hybridoma 31:225.
Product Citations: Volkova MV, et al. 2023. Int J Mol Sci. 24:. PubMed Wang L, et al. 2023. Pharm Biol. 61:568. PubMed El-Husseiny HM, et al. 2023. Int J Mol Sci. 24:. PubMed Yang F, et al. 2023. Mol Med Rep. 28:. PubMed Han X, et al. 2023. Stem Cells Int. 2023:8992284. PubMed Lan WR, et al. 2019. Stem Cells Int. 2019:8404168. PubMed Chou PR, et al. 2020. Int J Med Sci. 17:354. PubMed Qing P, et al. 2020. Exp Physiol. 1708:105. PubMed Shi Z, et al. 2020. Stem Cell Res Ther. 0.634027778. PubMed Sun J, et al. 2018. Exp Ther Med. 15:1424. PubMed Wei ZJ, et al. 2019. Neural Regen Res. 14:1462. PubMed
RRID: AB_2566265 (BioLegend Cat. No. 206105)
Structure: Ig superfamily, GPI-anchored membrane glycoprotein.
Distribution: Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, and glomeruli.
Function: Adhesion, signal transduction, lymphocyte co-stimulation, and proliferation and differentiation of hematopoietic stem cells.
Ligand/Receptor: Interacts with CD45.
Cell Type: B cells, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Campbell DG, et al. 1981. Biochem. J. 195:15. 2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.
Gene ID: 21838
UniProt: View information about CD90 on UniProt.org
Clone: 2E11
Regulatory Status: RUO
Other Names: Thy-1, Thy1
Isotype: Mouse IgG1, κ