Biolegend, 206108, Alexa Fluor® 647 anti-rat CD90 Antibody, 100μg

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, and stromal cells.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: PC12 cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality testedIHC-F – Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for relevant formats of this clone) include: immunoprecipitation and Western blotting.For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 206104) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg)
Application References(PubMed link indicates BioLegend citation): Kuroiwa K, et al. 2012. Hybridoma 31:225.
RRID: AB_2927848 (BioLegend Cat. No. 206107) AB_2927848 (BioLegend Cat. No. 206108)
Structure: Ig superfamily, GPI-anchored membrane glycoprotein.
Distribution: Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, and glomeruli.
Function: Adhesion, signal transduction, lymphocyte co-stimulation, and proliferation and differentiation of hematopoietic stem cells.
Ligand/Receptor: Interacts with CD45.
Cell Type: B cells, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Campbell DG, et al. 1981. Biochem. J. 195:15. 2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.
Gene ID: 21838
UniProt: View information about CD90 on UniProt.org
Clone: 2E11
Regulatory Status: RUO
Other Names: Thy-1, Thy1
Isotype: Mouse IgG1, κ