Biolegend, 206303, PE anti-rat CD314 (NKG2D) Antibody, 25μg

NKG2D is a lectin-like type II transmembrane protein also known as CD314. It is expressed on NK cells and a subset of T lymphocytes. NKG2D serves as a stimulatory receptor to activate NK cells via the non-covalently associated DAP10 or DAP12 adaptor. Rat NKG2D has been reported to induce the synthesis of IFN gamma and induce cytotoxicity in response to rat RAE1L and PRLT, RAE family members in rat. NKG2D is also involved in liver allograft rejection.
25μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat NKG2D fused with human Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2890732 (BioLegend Cat. No. 206303) AB_2890732 (BioLegend Cat. No. 206304)
Structure: A lectin-like type II transmembrane protein
Distribution: NK cells and a subset of T lymphocytes
Function: A stimulatory receptor to activate NK cells by association with DAP10 or DAP12 adaptor
Ligand/Receptor: RAE1L and PRLT
Cell Type: NK cells, NKT cells, T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors, Innate Immune Signaling
Antigen References: Obara H, et al. 2005. Am. J. Transplant. 5:2094-103. Raulet DH. 2003. Nat. Rev. Immunol. 3:781-790. Sun Z, et al. 2009. Hepatology. 49:587-597. Dissen E, et al. 1996. J. Exp. Med.183:2197-2207. Løvik G, et al. 1995. J. Immunol. Methods. 179:59-69. Ming Z, et al. 2010. Eur. J. Immunol. 40:1748-1757.
Gene ID: 24934
UniProt: View information about CD314 on UniProt.org
Clone: 11D5C9
Regulatory Status: RUO
Other Names: KLRK1, NKR2
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.