Biolegend, 250002, Purified anti-pig CD4a Antibody, 100μg

CD4 is a 55 kDa, type I glycoprotein expressed by tymocytes, helper T cells (Th), plasmacytoid dendritic cells (also known as natural interferon-producing cells or NIPC). In Pigs there a CD4+/CD8+ double positive subset that increase with age and have characteristics of memory T cells.
100μg
Verified Reactivity: Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Swine thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 74-12-4 recognizes the allele CD4.A in swine T cells.
Application References(PubMed link indicates BioLegend citation): Pescovitz, M.D. et al. 1984. J. Immunol. 133:368 (FC, IP) Pescovitz, M.D. et al. 1985. J. Immunol. 134:37 (FC) Saalmuller, A., et al. 1987. Eur. J. Immunol. 17:1297 (FC)
RRID: AB_2894685 (BioLegend Cat. No. 250002)
Structure: 55 kD, type I glycoprotein
Distribution: Thymocytes, helper T cells, plasmacytoid dendritic cells
Interaction: TCR/CD3 complex
Ligand/Receptor: MHC class II
Cell Type: T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Gustafsson K, et al. 1993. J Immunol. 151:1365-70. Blanc F, et al. 2018. Immunogenetics. 70:209-222. Zhang F, et al. 2019. Gene. 707:22-29.
Gene ID: 404704
UniProt: View information about CD4a on UniProt.org
Clone: 74-12-4
Regulatory Status: RUO
Other Names: T-cell surface antigen T4/Leu-3
Isotype: Mouse IgG2b, κ