Biolegend, 250204, PE anti-marmoset CD45 Antibody, 100μg

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
100μg
Verified Reactivity: Common Marmoset
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Marmoset mononuclear cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen spleen sections1 and Western blotting1. The 6C9 antibody has been reported to not cross-react with humans, cynomolgus monkeys, or squirrel monkeys.
Application References(PubMed link indicates BioLegend citation): Ito R, et al. 2008. Immunol. Lett. 121:116. (IHC, WB)
Product Citations: Gordeychuk IV, et al. 2019. Acta Naturae. 10:63. PubMed Ireland RE, et al. 2022. Viruses. 14:. PubMed Geirsdottir L, et al. 2020. Cell. 179(7):1609-1622.e16.. PubMed
RRID: AB_2562120 (BioLegend Cat. No. 250204)
Structure: Tyrosine phosphatases, type I transmembrane protein
Distribution: Hematopoietic cells, except circulating erythrocytes or platelets
Function: TCR and BCR mediated activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I and Thomas M. 1994. Annu. Rev. Immunol. 12:85.
Gene ID: 100385061
UniProt: View information about CD45 on UniProt.org
Clone: 6C9
Regulatory Status: RUO
Other Names: LCA, T200
Isotype: Mouse IgG1
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.