Biolegend, 250704, APC anti-rat NKG2A/C/E Antibody, 100μg

The NKG2 molecules are a family of lectin-like receptors that form heterodimers with CD94. NKG2/CD94 heterodimer are primarily expressed on NK cells, and a subset of CD8 + T cells. Studies of CD94/NKG2 heterodimers on NK cells have demonstrated that the NKG2 components transduce signals after ligand binding.  NKG2A transduces inhibitory signals, while NKG2C and NKG2E transduce activating signals.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant rat NKG2A-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Sasther PC, et al, 2011, J Immunol, 187:6365
Product Citations: Dodagatta-Marri E, et al. 2021. Cell Rep. 36:109309. PubMed
RRID: AB_2728228 (BioLegend Cat. No. 250704)
Structure: Type II transmembrane protein
Distribution: NK cells, a subset of CD8+ T-lymphocytes.
Function: Signal transduction, Receptor for non-classical MHC Class I molecules
Interaction: DAP10, DAP12, CD94
Ligand/Receptor: Non-classical MHC Class I glycoproteins
Cell Type: NK cells, T cells
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: Berg SF, et al. 1998. Eur J Immunol, 28:444. Vance RE, et al. 1999. J Exp Med. 190(12). Vance RE, et al. 1998. J Exp Med. 188(10). Flornes LM, et al. 2010, Immunogenetics, 62:521 Dissen E, et al. 2008. Semin Immunol. 20:369
Gene ID: 2968329684500338
UniProt: View information about NKG2A on UniProt.org
Clone: WEN28
Regulatory Status: RUO
Other Names: KLRC1, Killer Cell Lectin Like Receptor C1, KLRC2, Killer Cell Lectin Like Receptor C2, KLRC3, Killer Cell Lectin Like Receptor C3
Isotype: Mouse IgG1, κ