Biolegend, 260110, GMP PerCP anti-human CD45 Antibody, 100tests

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
Concentration: 200 &microg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17. It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Kishihara K, et al. 1993. Cell 74:143. Esser M, et al. 2001. J. Virol. 75:6173. (WB) Yamada T, et al. 2002. J. Biol. Chem. 277:28830. Nagano M, et al. 2007. Blood 110:151. Jiang Q, et al. 2008. Blood 112:2858. PubMed Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC) Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF) Lee J, et al. 2015. J Exp Med. 212:385. PubMed Breton G, et al. 2015. J Exp Med. 212:401. PubMed Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution: Hematopoietic cells, not expressed in circulating erythrocytes or platelets
Function: TCR and BCR mediated activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: Thomas M. 1989. Annu. Rev. Immunol. 7:339. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID: 5788
UniProt: View information about CD45 on UniProt.org
Clone: HI30
Other Names: LCA, T200
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.