Biolegend, 260170, GMP APC/Fire™ 750 anti-human HLA-DR Antibody, 100tests

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4 + T cells.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 200 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19 Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).
Application References(PubMed link indicates BioLegend citation): Brodsky F. 1984. Immunogenetics 19:179. Robbins P, et al. 1987. Human Immunol. 18:301. Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161. Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC) Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC) Zipf T, et al. 1981. Cancer Res. 41:4786. Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion) Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB) Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block) Wang RF, et al. 1999. Science 284:1351. (Block) Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC) Stein R, et al. 2011. Leuk. Lymphoma 52:273. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329. Moro M, et al. 2005. BMC Immunol. 6:24. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
Distribution: B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
Function: Peptide presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177. 2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138. 3. Edwards JA, et al. 1986. J. Immunol. 137:490. 4. van Es A, et al. 1984. Transplantation 37:65. 5. O'Doherty U, et al. 1994. Immunology 82:487. 6. Thomas R, et al. 1994. J. Immunol. 153:4016. 7. Grouard G, et al. 1996. Nature 384:364.
Gene ID: 31223123
UniProt: View information about HLA-DR on UniProt.org
Clone: L243
Other Names: Major Histocompatibility Class II, MHC class II
Isotype: Mouse IgG2a, κ