Biolegend, 260172, GMP Pacific Blue™ anti-human CD14 Antibody, 100tests

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full-length human CD14 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 200 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
Application References(PubMed link indicates BioLegend citation): McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F) Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Power CP, et al. 2004. J. Immunol. 173:5229. (Block) Williams KC, et al. 2001. J. Exp. Med. 193:905. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG) Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: GPI-linked membrane glycoprotein, 53-55 kD
Distribution: Monocytes, macrophages, granulocytes (low)
Function: LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor: LPS
Cell Type: Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Stocks S, et al. 1990. Biochem. J. 268:275. 2. Wright S, et al. 1990. Science 249:1434.
Gene ID: 929
UniProt: View information about CD14 on UniProt.org
Clone: M5E2
Workshop: III 329
Other Names: LPS receptor
Isotype: Mouse IgG2a, κ