Biolegend, 260312, GMP APC/Fire™ 750 anti-human CD5 Antibody, 100tests

CD5, also known as Leu-1, Ly-1 and T1, is a 67 kD single chain type I glycoprotein that is a member of the scavenger receptor superfamily. CD5 is expressed on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-CLL), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions. One of its ligands is CD72.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human T-acute lymphoblastic leukemia (ALL) cells
Formulation: Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 200 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats): immunoprecipitation1,3,4 and immmunohistochemical staining of frozen and formalin-fixed paraffin-embedded sections2,3,5.
Application References(PubMed link indicates BioLegend citation): McAlister MS, et al. 1998. Protein Eng. 11:847. (IP) Butmarc JR, et al. 1998. Am. J. Clin. Pathol. 109:682. (IHC) Engleman EG, et al. 1981. Proc. Natl. Acad. Sci. USA 78:1791. (IHC, IP) Ledbetter JA, et al. 1981. J. Exp. Med. 153:310. (FC, IP) Warnke R and Levy R. 1980. J. Histochem. Cytochem. 28:771. (IHC)
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Scavenger receptor superfamily, 67 kD
Distribution: Thymocytes, T cells, and subsets of B and dendritic cells.
Function: Modulates TCR and BCR signaling, involved in thymocyte maturation and T-B cell interaction
Ligand/Receptor: CD72
Cell Type: B cells, Dendritic cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Kipps T. 1989. Adv. Immunol. 47:117. 2. Resnick D, et al. 1994. Trends Biochem. Sci. 1:5. 3. Wood GS and Freudenthal PS. 1992. Am. J. Pathol. 141:789.
Gene ID: 921
UniProt: View information about CD5 on UniProt.org
Clone: L17F12
Workshop: II T7
Other Names: Leu-1, Ly-1, T1, Tp67, Leu1
Isotype: Mouse IgG2a, κ