Biolegend, 260472, GMP APC/Fire™ 750 anti-human CD24 Antibody, 100tests

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca 2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).
100tests
Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 400 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy3.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York. Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF) Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: GPI-linked glycoprotein, 35-45 kD
Distribution: B cells, granulocytes, epithelial cells
Function: B cell proliferation and differentiation
Ligand/Receptor: CD62P (P-Selectin)
Cell Type: B cells, Epithelial cells, Granulocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
Gene ID: 100133941
UniProt: View information about CD24 on UniProt.org
Clone: ML5
Workshop: V CD24.5
Other Names: Ly-52, Heat Stable Antigen (HSA), Nectadrin, BA-1
Isotype: Mouse IgG2a, κ