Biolegend, 260474, GMP FITC anti-human CD27 Antibody, 100tests

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.
100tests
Reactivity: Human
Reported Reactivity: Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human T cells from a T-ALL patient.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 200 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections1, immunofluorescent staining2, and ELISA3.
Application References(PubMed link indicates BioLegend citation): Ma S, et al. 2011. J. Virol. 85:165. (IHC) Manzo A, et al. 2008. Arthritis Rheum. 11:3377. (IF) Kato K, et al. 2007. Exp. Hematol. 35:434. (ELISA)
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution: Medullary thymocytes, T and B cell subsets, NK cells
Interaction: CD27 binds to CD70
Ligand/Receptor: CD70
Cell Type: B cells, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. 2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. 3. Hintzen R, et al. 1994. Immunol. Today 15:307. 4. Agematsu K, et al. 1995. J. Immunol. 154:3627.
Gene ID: 939
UniProt: View information about CD27 on UniProt.org
Clone: M-T271
Workshop: V 5T CD27.03
Other Names: S152, T14, TNFRSF7
Isotype: Mouse IgG1, κ