Biolegend, 260528, GMP APC anti-human CD9 Antibody, 100tests

CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β 1 integrins.
100tests
Reactivity: Human
Reported Reactivity: African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rabbit, Rhesus, Sheep
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 6 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Tetraspan family, type III transmembrane protein, 24 kD
Distribution: Platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial and epithelial cells
Function: Adhesion, platelet activation, B cell development
Ligand/Receptor: Associates with CD63, CD81, CD82 and CD36, binds PSG17
Cell Type: B cells, Embryonic Stem Cells, Endothelial cells, Epithelial cells, Granulocytes, Lymphocytes, Platelets
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Miao WM, et al. 2001. Blood 97:1689. 2. Ellerman DA, et al. 2003. Mol Biol Cell. 14:5098. 3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Gene ID: 928
UniProt: View information about CD9 on UniProt.org
Clone: HI9a
Workshop: V P018
Other Names: Tetraspanin, MRP-1, DRAP-24
Isotype: Mouse IgG1, κ