Biolegend, 285249, Spark Blue™ 550 anti-mouse/human KLRG1 (MAFA) (Flexi-Fluor™) Antibody, 50μg

Killer cell lectin-like receptor G1 (KLRG1) is the mouse homolog of the rat mast cell function-associated antigen (MAFA or 2F1-Ag).  KLRG1 is a type II membrane glycoprotein that was first identified on the surface of rat mast cell line RBL-2H3.  It is composed of a homodimer of glycosylated 30-38 kD subunits.  Mouse and human homologs of KLRG1 are expressed by subsets of NK cells and lymphokine-activated killer (LAK) cells but not mast cells.  KLRG1 is also expressed on subsets of CD8 + and CD4 + cells, including CD4 + and CD8 + effector/memory cells, potent regulatory CD4 + T cells.  KLRG1 may be involved in regulating NK cell homeostasis.  KLRG1 was found to recognize cadherins and thus inhibit immune responses by regulating the effector function and the developmental processes of NK and T cells.
50μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Immunogen: IL-2 activated NK cells from C57BL/6 mice
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL. For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. * Spark Blue™ 550 has a maximum excitation of 516 nm and a maximum emission of 540 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421™, or APC, are recommended.Additional reported applications (for relevant formats) include: Western Blotting1.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
Application References(PubMed link indicates BioLegend citation): Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB) Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC) McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
RRID: AB_3662553 (BioLegend Cat. No. 285249)
Structure: KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.
Distribution: Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8+ and CD4+ cells.
Function: Regulates NK cell proliferation, maturation, and homeostasis. Inhibits immune responses by regulating the effector function and the developmental processes of NK and T cells.
Ligand/Receptor: E-cadherin
Cell Type: NK cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Grundemann C, et al. 2006. J. Immunol. 176:1311. 2. Blaser C, et al. 1998. J. Immunol. 161:6451. 3. Huntington ND, et al. 2007. J. Immunol. 178:4764. 4. Voehringer D, et al. 2001. J. Immunol. 167:4834.
Gene ID: NA
UniProt: View information about KLRG1 on UniProt.org
Clone: 2F1/KLRG1
Regulatory Status: RUO
Other Names: MAFA, 2F1-Ag
Isotype: Syrian Hamster IgG
Q: What are Flexi-Fluors?
A: Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
Q: How quickly will I receive my order?
A: We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
Q: How are Flexi-Fluors different from regular catalog products?
A: Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
Q: How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
A: Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL. 

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody.  Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Q: I can’t find the antibody-dye combination that I need. When will it be available?
A: We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
Q: I need help to validate the performance of my Flexi-Fluor. Who should I contact?
A: Please get in touch with Technical Service for assistance.
Q: Can I order more than 50 μg of a Flexi-Fluor?
A: Yes, you can order multiple vials of the same Flexi-Fluor products.  We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
Q: What is the expiration date of my Flexi-Fluor?
A: Expiration dates can be found on the vial label or by using our CoA lookup tool.
Q: What if my requested antibody-dye combination doesn't perform as expected?
A: Flexi-Fluor™ products have been carefully selected by our scientists for their excellent conjugation properties and broad compatibility. In the rare case that an antibody-dye combination does not perform as expected due to the unique characteristics of a specific pairing, please contact our Technical Service team. We will work with you to identify alternative options to meet your panel requirements.