Biolegend, 300407, PE anti-human CD3 Antibody, 25tests

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
25tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP) Graves J, et al. 1991. J. Immunol. 146:2102. (Activ) Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ) Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC) Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ) Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC) Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC) Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Van Dongen JJM, et al. 1988. Blood 71:603. (WB) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC) Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed Li J, et al. 2015. Cancer Res. 75:508. PubMed Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Jenkins E, et al. 2023. Nat Commun. 14:1611. PubMed Merah-Mourah F, et al. 2021. Curr Protoc. 1:e229. PubMed Sefik E, et al. 2022. Nature. 606:585. PubMed Sušac L, et al. 2022. Cell. 185:3201. PubMed Groen R, et al. 2015. J Leukoc Biol. 98: 623 - 630. PubMed Miggitsch C, et al. 2019. EBioMedicine. 46:387. PubMed Shoda H, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1308-y. PubMed Jeon SB, et al. 2022. Int J Stem Cells. Online ahead of print. PubMed Bessy T, et al. 2021. J Cell Biol. 220:. PubMed O’Connor K, et al. 2016. Genes Immun. 10.1038/gene.2016.27. PubMed Thakral D, et al. 2008. J Immunol. 180:7431. PubMed Mineo M, et al. 2020. Molecular Cell. 78(6):1207-1223.e8. PubMed Sun B, et al. 2019. Cancer Immunol Res. 1.550694444. PubMed Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed Ramendra R, et al. 2021. Front Immunol. 12:694152. PubMed Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed Nabet BY, et al. 2020. Cell. 183(2):363-376.e13. PubMed Hsiao CC, et al. 2022. STAR Protoc. 3:101422. PubMed Sefik E, et al. 2021. Nat Biotechnol. . PubMed Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed Hirama T, et al. 2021. JCI Insight. 6:. PubMed Dong G, et al. 2020. Haematologica. . PubMed Wang X, et al. 2020. Exp Ther Med. 19:3698. PubMed Georg P, et al. 2022. Cell. 185:493. PubMed Zhao NQ, et al. 2020. PLoS One. 15:e0238347. PubMed Tomita K, et al. 2013. Neuroscience. 236:55. PubMed Singh KS, et al. 2021. Nature. 589:597. PubMed Yu W, et al. 2022. Front Cell Dev Biol. 10:928140. PubMed Lanz AL, et al. 2021. Cell Reports. 36(2):109375. PubMed Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed Yuan Z, et al. 2016. J Virol. 90: 7728 - 7739. PubMed Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed Tiwarekar V, et al. 2018. J Virol. 92:17. PubMed Mysore V, et al. 2021. Med (N Y). 2:1050. PubMed Deng W, et al. 2021. Emerg Microbes Infect. 10:1272. PubMed
RRID: AB_314061 (BioLegend Cat. No. 300407) AB_314062 (BioLegend Cat. No. 300408) AB_2564150 (BioLegend Cat. No. 300456) AB_2562047 (BioLegend Cat. No. 300441)
Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
Gene ID: 916
UniProt: View information about CD3 on UniProt.org
Clone: UCHT1
Regulatory Status: RUO
Workshop: III 471
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.