Biolegend, 300431, Pacific Blue™ anti-human CD3 Antibody, 100tests

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP) Graves J, et al. 1991. J. Immunol. 146:2102. (Activ) Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ) Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC) Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ) Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC) Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC) Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Van Dongen JJM, et al. 1988. Blood 71:603. (WB) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC) Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed Li J, et al. 2015. Cancer Res. 75:508. PubMed Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Liu Y, et al. 2023. Nat Commun. 14:2179. PubMed Schnizer C 2022. Frontiers in Immunology. 13:1095129. PubMed Yen M, et al. 2022. Cell. 185:1414. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Weymar GHJ, et al. 2022. Cell Rep. 40:111311. PubMed Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed Kerkman PF, Kempers AC 2016. Ann Rheum Dis. 75(12):2201-2207. PubMed Manser AR, et al. 2019. J Immunol. 203:2301. PubMed Yarzabek B et al. 2018. eLife. 7 pii: e34961. PubMed Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed Geng J, et al. 2018. Elife. 7:e36341. PubMed Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed Alter G, et al. 2020. Cell. 183(1):185-196.e14. PubMed Tjwa E, et al. 2011. J Hepatol. 54:209. PubMed Ye C, et al. 2017. J Virol. 91:e01389-23. PubMed Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed Fu W, et al. 2016. Sci Rep. 6:38162. PubMed Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed Bego M, et al. 2015. J Vis Exp. 103: 51207. PubMed Hesketh AJ et al. 2015. PloS one. 10(12):e0145197 . PubMed Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed Schoutrop E, et al. 2022. Oncoimmunology. 11:2093426. PubMed Gawel DR, et al. 2019. Genome Med. 11:47. PubMed Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed Horwitz JA, et al. 2017. Cell. 170:637. PubMed Schneider EH, et al. 2020. Naunyn Schmiedebergs Arch Pharmacol. 393:1251. PubMed Roy Chowdhury R, et al. 2018. Nature. 560:644. PubMed Rydbirk R, et al. 2019. Sci Rep. 9:7781. PubMed Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed Wang R, et al. 2016. Proc Natl Acad Sci U S A. 113: 11501 - 11506. PubMed Yang C, et al. 2019. Nat Commun. 10:3931. PubMed Assadi G, et al. 2016. PLoS One. 11:e0168276. PubMed Mock U, et al. 2015. Nucleic Acids Res. 43:5560. PubMed Richard J, et al. 2013. Virology. 443:248. PubMed Grant EJ, et al. 2018. Nat Commun. 9:5427. PubMed Artinger M, et al. 2021. Int J Mol Sci. 22: . PubMed
RRID: AB_493095 (BioLegend Cat. No. 300418) AB_1595437 (BioLegend Cat. No. 300431) AB_493094 (BioLegend Cat. No. 300417) AB_2562048 (BioLegend Cat. No. 300442)
Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
Gene ID: 916
UniProt: View information about CD3 on UniProt.org
Clone: UCHT1
Regulatory Status: RUO
Workshop: III 471
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ