Biolegend, 300707, Purified anti-human CD54 Antibody, 100μg

CD54, also known as ICAM-1, is a 85-110 kD type I transmembrane protein. It is expressed on activated endothelial cells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, and dendritic cells. The expression of ICAM-1 on the cell surface is potently upregulated by activation; a soluble form of ICAM-1 can be released from the cell surface. CD54 plays a role in cellular adhesion and is involved in inflammation and leukocyte extravasation. CD54 has also been shown to be the major cellular receptor for rhinovirus. ICAM-1 binds to CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) as well as hyaluronan and fibrinogen.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Transfectant cells (aa28-480) and recombinant protein (aa28-480)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Flow cytometry testing showed that clone W21128D can block the binding of clones HA58 and HCD54 on target cells.
RRID: AB_3683299 (BioLegend Cat. No. 300707)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells. Cell surface expression upregulated upon activation; soluble ICAM-1 released from surface.
Function: Cellular adhesion, involved in inflammation and immune reactions. CD54 expressed on endothelial cells in areas of inflammation plays a role in leukocyte extravasation. Major cellular receptor for rhinovirus.
Ligand/Receptor: CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) and has also been reported to bind hyaluronan and fibrinogen.
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Voraberger G, et al. 1991 J Immunol. 147:2777-86. Staunton DE, et al. 1988. Cell. 52:925-33. Greve JM, et al. 1989. Cell. 56:839-47.
Gene ID: 3383
UniProt: View information about CD54 on UniProt.org
Clone: W21128D
Regulatory Status: RUO
Other Names: ICAM-1, Ly-47
Isotype: Rat IgG1, κ