Biolegend, 300818, Brilliant Violet 785™ anti-human CD137 (4-1BB) Antibody, 100tests

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD137-Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_3097264 (BioLegend Cat. No. 300817) AB_3097264 (BioLegend Cat. No. 300818)
Structure: TNFR superfamily, type I transmembrane protein, 30 kD
Distribution: Activated T cells
Function: T cell costimulation
Ligand/Receptor: 4-1BB ligand (CD137L)
Cell Type: Lymphocytes, NK cells, NKT cells, T cells
Biology Area: Costimulatory Molecules, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family: CD Molecules
Antigen References: Gruss HJ & Dower SK. 1995. Blood. 85:3378-404. Sica G & Chen L. 2000. Adv Exp Med Biol. 465:355-62. Alderson MR, et al. 1994. Eur J Immunol. 24:2219-27. Schwarz H, et al. 1996. Blood 87:2839-45.
Gene ID: 3604
UniProt: View information about CD137 on UniProt.org
Clone: S18014C
Regulatory Status: RUO
Other Names: 4-1BB, ILA, CDw137, TNFRSF9
Isotype: Mouse IgG1, κ