Biolegend, 301411, PE/Cyanine7 anti-human CD11b (activated) Antibody, 25tests

The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as α M integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β 2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils as well as adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and factor X.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the a chain. Clone CBRM1/5 binds the I domain, close to the ligand binding site. It recognizes a conformational change of the integrin9. Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking cell adhesion to fibrinogen and ICAM-11. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301408).
Application References(PubMed link indicates BioLegend citation): Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block) Bryn T, et al. 2006. J. Immunol. 176:7361. PubMed Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200. Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed Koleva RI, et al. 2012. Blood. 119:4878. PubMed. Eleftherious D, et al. 2012. Neurology. 79:2089. PubMed Oxvig C, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2215. Muller-Edenborn B, et al. 2014. Br J Anaesth. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Product Citations: Zheng QY, et al. 2019. Front Immunol. 10:1866. PubMed Zheng QY, et al. 2020. FASEB J. 34:10590. PubMed
RRID: AB_2563571 (BioLegend Cat. No. 301411) AB_2563572 (BioLegend Cat. No. 301412)
Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, T cell subset, B cell subset
Function: Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Stewart M, et al. 1995. Curr. Opin. Cell Biol. 7:690. 2. Petty H, et al. 1996. Immunol. Today 17:209.
Gene ID: 3684
UniProt: View information about CD11b on UniProt.org
Clone: CBRM1/5
Regulatory Status: RUO
Other Names: Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype: Mouse IgG1, κ