Biolegend, 301724, Ultra-LEAF™ Purified anti-human CD13 Antibody, 1mg

CD13 is a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. This zinc metallopeptidase is expressed as a homodimer on granulocytes, myeloid progenitors, endothelial cells, epithelial cells and subset of granular lymphoid cells. It is not expressed on platelets or erythrocytes. CD13 is thought to be involved in the metabolism of many regulatory peptides and functions in antigen processing and the cleavage of chemokines such as MIP-1. CD13 serves as the cellular receptor for Coronavirus.
1mg
Verified Reactivity: Human
Reported Reactivity: Baboon, Chimpanzee, Cotton-topped Tamarin
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: inhibition of tumor-cell invasion and blocking of aminopeptidase activities2,3, and immunohistochemical staining of acetone-fixed frozen tissue sections5. WM15 does not recognize formalin-fixed or paraffin-embedded tissue sections5. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301708). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301723 and 301724) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Saiki I, et al. 1993. Int J Cancer. 54:137. (Block) Rosenzwajg M, et al. 2000. Blood 95:453. (Block) Kawase M, et al. 2008. J Virol. 83:712. (Block) PubMed Di Matteo P, et al. 2011. J. Histochem. Cytochem. 59:47. (IHC)
RRID: AB_2728236 (BioLegend Cat. No. 301723) AB_2728237 (BioLegend Cat. No. 301724)
Structure: Zinc metallopeptidase, type II integral membrane glycoprotein, 150-170 kD
Distribution: Granulocytes, monocytes, myeloid progenitors, endothelial and epithelial cells, granular lymphocyte subset
Function: Zinc-binding metalloproteinase, antigen processing, cleaves MIP-1 chemokine
Ligand/Receptor: Coronavirus receptor
Cell Type: Endothelial cells, Epithelial cells, Granulocytes, Hematopoietic stem and progenitors, Lymphocytes, Mesenchymal Stem Cells, Monocytes, Neutrophils
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Shipp M, et al. 1993. Blood 82:1052. 2. Larsen S, et al. 1996. J. Exp. Med. 184:183.
Gene ID: 290
UniProt: View information about CD13 on UniProt.org
Clone: WM15
Regulatory Status: RUO
Workshop: IV M44
Other Names: Aminopeptidase N, APN, gp150, ANPEP
Isotype: Mouse IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.