Biolegend, 302056, PE anti-human CD16 Antibody, 100μg

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
100μg
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PMN cells
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP) Hoshino S, et al. 1991. Blood 78:3232. (Stim) Tamm A, et al. 1996. Immunol. 157:1576. (Block) Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC) Holl V, et al. 2004. J. Immunol. 173:6274. (Block) Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block) Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Rout N, et al. 2010. PLoS One 5:e9787. (FC) Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC) Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed Wu Z, et al. 2013. J. Virol. 87:7717. PubMed Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG) Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
Product Citations: Parasar P, et al. 2022. Am J Reprod Immunol. 88:e13614. PubMed Zhou J, et al. 2023. Front Endocrinol (Lausanne). 14:1069395. PubMed Zhang Y, et al. 2023. Heliyon. 9:e14823. PubMed Wang T, et al. 2022. Front Immunol. 13:954121. PubMed Subedi N, et al. 2022. Adv Biol (Weinh). :e2200207. PubMed Ismailova A, et al. 2023. Front Immunol. 14:1123344. PubMed Zong D, et al. 2021. BMC Biol. 19:79. PubMed Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed Parameswaran R, et al. 2016. Nat Commun. 7: 11154. PubMed Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed Ostendorf L, et al. 2019. Front Immunol. 2.309027778. PubMed Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed Devalraju KP, et al. 2021. PLoS One. 16:e0257185. PubMed Drummer DJ, et al. 2022. Int J Exerc Sci. 15:686. PubMed Nguyen D, et al. 2006. Exp Hematol. 34:728. PubMed Woolsey C et al. 2019. Cell Rep. 28(12):3032-3046 . PubMed Song S, et al. 2021. Commun Biol. 4:1338. PubMed Jankeel A, et al. 2020. J Virol. :94. PubMed Subedi N, et al. 2021. Sci Rep. 11:17084. PubMed Murdock BJ, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed Balan I, et al. 2022. Front Immunol. 13:940095. PubMed Huang SSY, et al. 2021. Biology. 10(8):. PubMed Schrøder M, et al. 2016. PLoS One. 11: 0157387. PubMed Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed Crifò B, et al. 2019. J Immunol. 202:1521. PubMed Hakimi AA, et al. 2019. Cancer Discov. 9:510. PubMed Reichwald K, et al. 2014. PLoS One. 9:85793. PubMed Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed Yeo L, et al. 2018. J Clin Invest. 128:3460. PubMed LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed Radom-Aizik S, et al. 2014. Brain Behav Immun. 39:121. PubMed Yang X, et al. 2019. Mol Med Rep. 19:1083. PubMed Karantanos T, et al. 2022. Sci Adv. 8:eabl8952. PubMed Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed Maharaj S, et al. 2018. Nitric Oxide. 72:41:00. PubMed Walle T, et al. 2022. Sci Adv. 8:eabh4050. PubMed Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed Siegers G, et al. 2011. PLoS One. 6:e16700. PubMed
RRID: AB_314207 (BioLegend Cat. No. 302007) AB_314208 (BioLegend Cat. No. 302008) AB_2564139 (BioLegend Cat. No. 302056)
Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution: NK cells, activated monocytes, macrophages, neutrophils
Function: Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor: Aggregated IgG, IgG-antigen complex
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275. 2. Stroncek D, et al. 1991. Blood 77:1572. 3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.
Gene ID: 2214
UniProt: View information about CD16 on UniProt.org
Clone: 3G8
Regulatory Status: RUO
Workshop: V NK80
Other Names: FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype: Mouse IgG1, κ
Q: Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?
A: Yes
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.