Biolegend, 302102, Purified anti-human CD18 Antibody, 100μg

CD18 is a 90-95 kD type I transmembrane protein also known as integrin β 2 subunit, LFA-1 β subunit, and β 2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.
100μg
Verified Reactivity: Human
Reported Reactivity: Pig
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 302116) is recommended for functional assays.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto T, et al. 1997. Leucocyte Typing VI. Garland Press. London. Van Epps DE, et al. 1989. J. Immunol. 143:3207. (Block) Meerschaert J, et al. 1994. J. Immunol. 152:1915. (Block) Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200. Sommaggio R, et al. 2012. J. Immunol. 188:2075. PubMed Valenzuela NM, et al. 2013. J. Immunol. 190:6635. PubMed
Product Citations: Mascarau R, et al. 2023. J Cell Biol. 222:. PubMed Kirolos SA, et al. 2022. J Leukoc Biol. 112:1399. PubMed Gong YY, et al. 2022. Cancer Res Commun. 2:842. PubMed Zhang W, et al. 2022. Dev Cell. 57:329. PubMed Hosoki K, et al. 2012. J Leukoc Biol. 92:333. PubMed Nakayama H, et al. 2008. J Leukoc Biol. 83:728. PubMed Sommaggio R, et al. 2012. J Immunol. 188:2075. PubMed Tran D, et al. 2009. J Immunol. 182:2929. PubMed Kolev M, et al. 2020. Immunity. 52(3):513-527. PubMed Chakrabarty S, et al. 2011. Blood. 117:1585. PubMed
RRID: AB_314220 (BioLegend Cat. No. 302102)
Structure: Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD
Distribution: Leukocytes
Function: Adhesion and signaling in hematopoietic system
Ligand/Receptor: ICAMs
Cell Type: Leukocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175. 2. Springer T. 1994. Cell 76:301.
Gene ID: 3689
UniProt: View information about CD18 on UniProt.org
Clone: TS1/18
Regulatory Status: RUO
Workshop: V AS162
Other Names: Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2
Isotype: Mouse IgG1, κ