Biolegend, 302118, PE/Cyanine7 anti-human CD18 Antibody, 100tests

CD18 is a 90-95 kD type I transmembrane protein also known as integrin β 2 subunit, LFA-1 β subunit, and β 2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.
100tests
Verified Reactivity: Human
Reported Reactivity: Pig
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 302116) is recommended for functional assays.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto T, et al. 1997. Leucocyte Typing VI. Garland Press. London. Van Epps DE, et al. 1989. J. Immunol. 143:3207. (Block) Meerschaert J, et al. 1994. J. Immunol. 152:1915. (Block) Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200. Sommaggio R, et al. 2012. J. Immunol. 188:2075. PubMed Valenzuela NM, et al. 2013. J. Immunol. 190:6635. PubMed
Product Citations: Kolev M, et al. 2020. Immunity. 52(3):513-527. PubMed
RRID: AB_2565584 (BioLegend Cat. No. 302117) AB_2565585 (BioLegend Cat. No. 302118)
Structure: Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD
Distribution: Leukocytes
Function: Adhesion and signaling in hematopoietic system
Ligand/Receptor: ICAMs
Cell Type: Leukocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175. 2. Springer T. 1994. Cell 76:301.
Gene ID: 3689
UniProt: View information about CD18 on UniProt.org
Clone: TS1/18
Regulatory Status: RUO
Workshop: V AS162
Other Names: Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2
Isotype: Mouse IgG1, κ