Biolegend, 302601, Purified anti-human CD25 Antibody, 25μg

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4 + T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
25μg
Verified Reactivity: Human
Reported Reactivity: Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications include: immunocytochemistry3.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations: Spoerl S, et al. 2021. Eur J Immunol. 51:1436. PubMed Alpert A, et al. 2022. Cell Syst. 13:71. PubMed Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed Wang W, et al. 2018. Cancer Cell. 34:757. PubMed Volchenkov R, et al. 2017. Front Immunol. 0.818055556. PubMed Chakhtoura M, et al. 2021. PLoS Pathog. 17:e1009732. PubMed Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed Li H, et al. 2020. Front Immunol. 11:1777. PubMed Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed Ardain A, et al. 2019. Nature. 570:528. PubMed Martin E, et al. 2020. JCI Insight. :5. PubMed Umeda M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed Chiou SH, et al. 2021. Immunity. 54:586. PubMed
RRID: AB_314272 (BioLegend Cat. No. 302602)
Structure: Type I transmembrane glycoprotein, 55 kD
Distribution: Activated T cells and B cells, monocytes/macrophages, Treg
Function: Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor: IL-2
Cell Type: B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
Gene ID: 3559
UniProt: View information about CD25 on UniProt.org
Clone: BC96
Regulatory Status: RUO
Workshop: V T-072
Other Names: Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype: Mouse IgG1, κ