Biolegend, 302612, PE/Cyanine7 anti-human CD25 Antibody, 100tests

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4 + T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
100tests
Verified Reactivity: Human
Reported Reactivity: Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications include: immunocytochemistry3.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations: Couturier J, et al. 2016. Retroviology. 13:30. PubMed Gkika E, et al. 2023. NPJ Precis Oncol. 7:24. PubMed Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed Krammer S, et al. 2022. Cells. 12: . PubMed Vondra S, et al. 2023. Cell Rep. 42:111977. PubMed Veatch JR, et al. 2022. Cancer Cell. 40:393. PubMed Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed Barry KC, et al. 2018. Nat Med. 24:1178. PubMed Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed Massafra V, et al. 2021. J Immunol. 207:493. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Wu W, et al. 2020. Oncol Lett. 0.91875. PubMed Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed Gorczynski RM, et al. 2017. Immunology. 150:418. PubMed Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed Levring TB, et al. 2019. Sci Rep. 9:16725. PubMed Sayin I, et al. 2018. J Exp Med. 7:40286. PubMed Acharya A, et al. 2020. J Virol. 95:e01657. PubMed Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed Xu X, et al. 2020. Arthritis Rheumatol. 72:1303. PubMed NULL, et al. 2022. Cell. 185:916. PubMed Weinberg A, et al. 2015. PLoS One. 10:122431. PubMed Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
RRID: AB_314281 (BioLegend Cat. No. 302611) AB_314282 (BioLegend Cat. No. 302612)
Structure: Type I transmembrane glycoprotein, 55 kD
Distribution: Activated T cells and B cells, monocytes/macrophages, Treg
Function: Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor: IL-2
Cell Type: B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
Gene ID: 3559
UniProt: View information about CD25 on UniProt.org
Clone: BC96
Regulatory Status: RUO
Workshop: V T-072
Other Names: Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype: Mouse IgG1, κ