Biolegend, 302614, APC/Cyanine7 anti-human CD25 Antibody, 100tests

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4 + T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
100tests
Verified Reactivity: Human
Reported Reactivity: Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications include: immunocytochemistry3.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations: Tunali G, et al. 2023. J Clin Invest. :. PubMed Arra A, et al. 2021. Oncoimmunology. 10:1938475. PubMed Arunachalam PS, et al. 2021. Nature. 596:410. PubMed Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed Fletcher H, et al. 2016. Nat Commun. 7: 11290. PubMed Muller L, et al. 2017. Oncoimmunology. 6:e1261243. PubMed Stevenson EM, et al. 2022. Nat Commun. 13:4888. PubMed Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed Smith AS, et al. 2022. J Immunother Cancer. 10:. PubMed Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed Liu T, et al. 2022. Front Immunol. 13:901349. PubMed Arcangeli S, et al. 2022. J Clin Invest. 132: . PubMed Cornwell W, et al. 2013. J Neuroimmunol. 265:43. PubMed Muller L, et al. 2016. Sci Rep. 6:20254. PubMed Gawel DR, et al. 2019. Genome Med. 11:47. PubMed Sam J, et al. 2020. Front Oncol. 10:575737. PubMed Corrado M, et al. 2020. Cell Metab. 32:981. PubMed Shan L, et al. 2017. Immunity. 47:766. PubMed
RRID: AB_314283 (BioLegend Cat. No. 302613) AB_314284 (BioLegend Cat. No. 302614)
Structure: Type I transmembrane glycoprotein, 55 kD
Distribution: Activated T cells and B cells, monocytes/macrophages, Treg
Function: Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor: IL-2
Cell Type: B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
Gene ID: 3559
UniProt: View information about CD25 on UniProt.org
Clone: BC96
Regulatory Status: RUO
Workshop: V T-072
Other Names: Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype: Mouse IgG1, κ