Biolegend, 302701, Purified anti-human CD26 Antibody, 25μg

CD26 is a 110 kD type II membrane protein also known as ADA-binding protein and dipeptidyl peptidase IV (DPPIV). It is a member of the peptidase and ectoenzyme family. CD26 is expressed on the membrane of mature thymocytes, T lymphocytes (upregulated upon activation), B cells, NK cells, and macrophages. CD26 cleaves off N-terminal X-Pro and X-Ala dipeptides from polypeptides. It plays an integral role as a costimulatory molecule in T cell activation. CD26 may interact with extracellular matrix proteins such as fibronectin or collagen, CD45 and ADA.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Press. London. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations: Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed van der Heide V, et al. 2022. Cell Rep. :110508. PubMed Liu S, et al. 2021. Cell Rep Med. 2:100411. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Henrick BM, et al. 2021. Cell. . PubMed Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID: AB_314286 (BioLegend Cat. No. 302702)
Structure: Peptidases and ectoenzyme families, type II glycoprotein, 110 kD
Distribution: Thymocyte subset, memory T cells, B cells, NK cells, epithelial cells, macrophages
Function: Dipeptidyl peptidase, T cell costimulation, HIV entry
Ligand/Receptor: Adenosine-deaminase, collagen
Cell Type: B cells, Epithelial cells, Macrophages, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Kameoka J, et al. 1993. Science 261:466. 2. Dang N, et al. 1990. J. Exp. Med. 172:649.
Gene ID: 1803
UniProt: View information about CD26 on UniProt.org
Clone: BA5b
Regulatory Status: RUO
Workshop: VI N-L078
Other Names: ADA-binding protein, DPP IV ectoenzyme, EC 3.4.14.5, DPP4
Isotype: Mouse IgG2a, κ