Biolegend, 302705, PE anti-human CD26 Antibody, 25tests

CD26 is a 110 kD type II membrane protein also known as ADA-binding protein and dipeptidyl peptidase IV (DPPIV). It is a member of the peptidase and ectoenzyme family. CD26 is expressed on the membrane of mature thymocytes, T lymphocytes (upregulated upon activation), B cells, NK cells, and macrophages. CD26 cleaves off N-terminal X-Pro and X-Ala dipeptides from polypeptides. It plays an integral role as a costimulatory molecule in T cell activation. CD26 may interact with extracellular matrix proteins such as fibronectin or collagen, CD45 and ADA.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Press. London. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations: Cadosch D, et al. 2010. Injury. 41:4. PubMed Haugstøyl ME, et al. 2023. Front Immunol. 14:1130370. PubMed Hu Y, et al. 2023. Front Immunol. 14:1128423. PubMed Demchenko A, et al. 2023. Int J Mol Sci. 24:. PubMed Seo HR, et al. 2022. Int J Mol Sci. 23: . PubMed Dong G 2023. Cell Metabolism. N/A. PubMed Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed Jacob A, et al. 2020. Nature Protocols. 14(12):3303-3332.. PubMed Scheuplein V, et al. 2015. J Virol. 89:3859. PubMed Alysandratos KD, et al. 2021. Cell Reports. 36(9):109636. PubMed Arda HE et al. 2018. Cell systems. 7(3):310-322 . PubMed Kohli A, et al. 2022. Life Sci Alliance. 5:. PubMed Berical A, et al. 2022. Nat Commun. 13:4270. PubMed McCauley KB, et al. 2018. Curr Protoc Stem Cell Biol. 45:e51. PubMed Hirai H, et al. 2022. Mol Ther Methods Clin Dev. 24:11. PubMed Worthen CA, et al. 2020. J Invest Dermatol. 140:2515. PubMed Suzuki S, et al. 2021. STAR Protocols. 2:100683. PubMed Huang J, et al. 2020. Cell Stem Cell. 27(6):962-973.e7. PubMed Wang S, et al. 2010. Nephrol Dial Transplant. 26:2144. PubMed Mithal A, et al. 2020. Nat Commun. 0.607638889. PubMed Spitalieri P, et al. 2022. Cells. 11:. PubMed Hawkins FJ, et al. 2020. Cell Stem Cell. 28(1):79-95.e8. PubMed O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed Yoon YJ, et al. 2022. Nat Commun. 13:3291. PubMed Jacob A, et al. 2017. Cell Stem Cell. 1.202777778. PubMed
RRID: AB_314289 (BioLegend Cat. No. 302705) AB_314290 (BioLegend Cat. No. 302706)
Structure: Peptidases and ectoenzyme families, type II glycoprotein, 110 kD
Distribution: Thymocyte subset, memory T cells, B cells, NK cells, epithelial cells, macrophages
Function: Dipeptidyl peptidase, T cell costimulation, HIV entry
Ligand/Receptor: Adenosine-deaminase, collagen
Cell Type: B cells, Epithelial cells, Macrophages, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Kameoka J, et al. 1993. Science 261:466. 2. Dang N, et al. 1990. J. Exp. Med. 172:649.
Gene ID: 1803
UniProt: View information about CD26 on UniProt.org
Clone: BA5b
Regulatory Status: RUO
Workshop: VI N-L078
Other Names: ADA-binding protein, DPP IV ectoenzyme, EC 3.4.14.5, DPP4
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.