Biolegend, 302901, Purified anti-human CD28 Antibody, 25μg

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
25μg
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F, Costim, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Nunes J, et al. 1993. Biochem. J. 293:835. Calea-Lauri J, et al. 1999. J. Immunol. 163:62. Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC) Marti F, et al. 2001. J. Immunol. 166:197. (Costim) Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim) Rivollier A, et al. 2004. Blood 104:4029. (Costim) Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim) Sheng W, et al. 2007.Elsevier 580:6819. PubMed Mitsuhashi M. 2007. Clin Chem.53:148. PubMed Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed Yoshino N, et al. 2008. Exp. Anim. (Tokyo) 49:97. (FC) Berg M, et al. 2008. J Leukoc Biol. 83:853. (IP) PubMed Rout N, et al. 2010. PLoS One 5:e9787. (FC) Leonard JA, et al. 2011. J. Virol. 85:6867. PubMed Nomura T, et al. 2012. J. Virol. 86:6481. PubMed
Product Citations: Liu L, et al. 2016. Nat Biotechnol. 10.1038/nbt.3461. PubMed Panigrahi S, et al. 2020. PLoS Pathog. e1008885:16. PubMed Mousset CM, et al. 2018. Oncoimmunology. 7:e1488565. PubMed Mitsuhashi M 2007. Clin Chem. 53:148. PubMed Duan LJ, et al. 2022. Cell Rep. 40:111284. PubMed Chen L, et al. 2023. Immunology. 169:204. PubMed Xiao C, et al. 2023. Nat Aging. 3:418. PubMed Meadows AM, et al. 2023. iScience. 26:106578. PubMed Menevse AN, et al. 2023. Acta Neuropathol Commun. 11:75. PubMed Eberhardt F, et al. 2023. Int J Mol Med. 52:. PubMed Mammadli M, et al. 2021. Clin Transl Med. 11:e625. PubMed Kim SJ, et al. 2020. J Biol Chem. 296:100108. PubMed Alpert A, et al. 2022. Cell Syst. 13:71. PubMed Saito T, et al. 2022. ACS Chem Biol. 17:217. PubMed Toothaker JM, et al. 2022. Development. 149: . PubMed Deepak HB, et al. 2022. Indian J Pharmacol. 54:183. PubMed Kim HS, et al. 2023. Bioact Mater. 21:241. PubMed Onnis A, et al. 2023. J Exp Med. 220: . PubMed Chai H, et al. 2023. Nat Commun. 14:213. PubMed Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed Mohamed H, et al. 2023. Biomedicines. 11: . PubMed Schnizer C 2022. Frontiers in Immunology. 13:1095129. PubMed Davé E, et al. 2023. MAbs. 15:2160229. PubMed Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed Giles JR, et al. 2022. Immunity. 55:557. PubMed Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Zhang Y, et al. 2019. Cell Res. 29:609. PubMed Li G, et al. 2021. Oncoimmunology. 10:1983306. PubMed Barzilai S, et al. 2017. Cell Rep. 18(3):685-699. PubMed Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed Zhu Y, et al. 2019. Front Genet. 10:141. PubMed Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed Liang J, et al. 2017. Nat Commun. 8:15732. PubMed Zucchetti AE, et al. 2019. Nat Commun. 10:2864. PubMed Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed Bluhm J, et al. 2018. Mol Ther. 26:1906. PubMed Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed Xiao C, et al. 2022. iScience. 25:103934. PubMed Tocheva AS, et al. 2020. J Biol Chem. 295:18036. PubMed Haas R, et al. 2015. PLoS Biol. 13: 1002202. PubMed Bobardt M, et al. 2020. PLoS One. 15:e0227715. PubMed Willimsky G, et al. 2021. Elife. 10:. PubMed Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed Peng Q, et al. 2022. EBioMedicine. 77:103904. PubMed Nathan A, et al. 2021. Cell. 184(17):4401-4413.e10. PubMed Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed Ye Z, et al. 2008. Infect Immun. 76:2541. PubMed Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed Holbrook AK, et al. 2019. Physiol Rep. 7:e14234. PubMed Dias J, et al. 2018. Proc Natl Acad Sci U S A. 115:E11513. PubMed Oberg HH, et al. 2020. J Leukoc Biol. 107:1081. PubMed Gaa R, et al. 2021. MAbs. 13:1978130. PubMed Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed Kondo H, et al. 2022. Front Immunol. 13:836923. PubMed Hu X, et al. 2021. British Journal of Pharmacology. 178(6):1445-1458. PubMed Li L, et al. 2020. J Transl Med. 18:363. PubMed Meléndez AV, et al. 2022. Cell Mol Life Sci. 79:513. PubMed Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed Qiu C, et al. 2022. Front Immunol. 12:764949. PubMed Guo T, et al. 2018. J Immunol. 200:500. PubMed Jin H, et al. 2019. MBio. 10:e01769-19. PubMed Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed Li H, et al. 2020. Front Immunol. 11:1777. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Cai J, et al. 2021. eLife. 10:00. PubMed Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed Ito T, et al. 2021. Commun Biol. 4:694. PubMed Liu Y, et al. 2022. Micromachines (Basel). 13:. PubMed Leonard J, et al. 2011. J Virol. 85:6867. PubMed Seo N, et al. 2018. Nat Commun. 9:435. PubMed Qiu H, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.08.036. PubMed Jakob M, et al. 2020. Cells. 10:. PubMed Tocheva AS, et al. 2020. J Biol Chem. 295:18036. PubMed Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed Jin Y, et al. 2017. Int Immunopharmacol. . 10.1016/j.intimp.2017.10.009. PubMed Pardons M, et al. 2019. PLoS Pathog. 15:e1007619. PubMed Gee MH, et al. 2018. Cell. 172:549. PubMed Wiedeman AE, et al. 2020. J Clin Invest. 130:480. PubMed Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed Bonavia AS, et al. 2022. Front Immunol. 13:940030. PubMed Levin SD, et al. 2020. Front Immunol. 10:3086. PubMed Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed Pawlak EN, et al. 2018. Retrovirology. 15:6. PubMed Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed Tsai HI, et al. 2021. EMBO Mol Med. 13:e12834. PubMed Duque JSR, et al. 2022. Nat Commun. 13:3700. PubMed Pan Y, et al. 2021. Br J Pharmacol. 178:4907. PubMed Sheng WY, et al. 2006. FEBS Lett. 580:6819. PubMed Moosmann C, et al. 2022. STAR Protoc. 3:101031. PubMed Cassioli C, et al. 2021. Front Cell Dev Biol. 9:673446. PubMed Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed Mazer M, et al. 2019. J Immunol. 203:2088. PubMed Nomura T, et al. 2012. J Virol. 86:6481. PubMed Naranbhai V, et al. 2022. Cell. . PubMed Deng J, et al. 2021. J Leukoc Biol. 110:1171. PubMed Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed He Y, et al. 2021. eLife. 10:00. PubMed Jones N, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01516. PubMed Bantug GR, et al. 2018. Immunity. 48:542. PubMed Ren X, et al. 2021. Lab Chip. 21:1527. PubMed Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed Martin E, et al. 2020. JCI Insight. :5. PubMed Umeda M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID: AB_314303 (BioLegend Cat. No. 302901) AB_314304 (BioLegend Cat. No. 302902)
Structure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution: Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
Function: T cell costimulation
Ligand/Receptor: CD80, CD86
Cell Type: B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. June CH, et al. 1994. Immunol. Today 15:321. 3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.
Gene ID: 940
UniProt: View information about CD28 on UniProt.org
Clone: CD28.2
Regulatory Status: RUO
Workshop: V-CD28.05
Other Names: T44, Tp44
Isotype: Mouse IgG1, κ