Biolegend, 302954, Alexa Fluor® 647 anti-human CD28 Antibody, 100tests

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
100tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Nunes J, et al. 1993. Biochem. J. 293:835. Calea-Lauri J, et al. 1999. J. Immunol. 163:62. Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC) Marti F, et al. 2001. J. Immunol. 166:197. (Costim) Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim) Rivollier A, et al. 2004. Blood 104:4029. (Costim) Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim) Sheng W, et al. 2007.Elsevier 580:6819. PubMed Mitsuhashi M. 2007. Clin Chem.53:148. PubMed Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed Yoshino N, et al. 2008. Exp. Anim. (Tokyo) 49:97. (FC) Berg M, et al. 2008. J Leukoc Biol. 83:853. (IP) PubMed Rout N, et al. 2010. PLoS One 5:e9787. (FC) Leonard JA, et al. 2011. J. Virol. 85:6867. PubMed Nomura T, et al. 2012. J. Virol. 86:6481. PubMed
RRID: AB_2721412 (BioLegend Cat. No. 302953) AB_2721413 (BioLegend Cat. No. 302954)
Structure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution: Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
Function: T cell costimulation
Ligand/Receptor: CD80, CD86
Cell Type: B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. June CH, et al. 1994. Immunol. Today 15:321. 3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.
Gene ID: 940
UniProt: View information about CD28 on UniProt.org
Clone: CD28.2
Regulatory Status: RUO
Workshop: V-CD28.05
Other Names: T44, Tp44
Isotype: Mouse IgG1, κ