Biolegend, 303119, APC/Cyanine7 anti-human CD31 Antibody, 25tests

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.
25tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF) Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block) Nagano M, et al. 2007. Blood 110:151. (FC) PubMed MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed Wu J, et al. 2022. Nat Commun. 13:676. PubMed Wu X, et al. 2022. Front Immunol. 13:811551. PubMed Zarobkiewicz M, et al. 2022. J Clin Med. 11: . PubMed Flaskamp L, et al. 2022. Cells. 11: . PubMed Basil MC, et al. 2022. Nature. 604:120. PubMed Lin GL, et al. 2018. Acta Neuropathol Commun. 9:617. PubMed Webster K, et al. 2013. Hum Reprod. 28:651. PubMed Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed Philippeos C, et al. 2018. J Invest Dermatol. 138:811. PubMed Panciera T, et al. 2020. Nat Mater. 1.345138889. PubMed Kathiriya JJ, et al. 2022. Nat Cell Biol. 24:10. PubMed Piekarska K, et al. 2022. Nat Commun. 13:856. PubMed Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed Spearman AD, et al. 2021. Front Pediatr. 9:679572. PubMed Wu X, et al. 2018. J Immunol Methods. 455:71. PubMed Xi H, et al. 2020. Cell Stem Cell. 27(1):158-176. PubMed Phansalkar R, et al. 2021. Elife. 10:. PubMed Xu J et al. 2019. Elife. 8 pii: e48191. PubMed Camunas–Soler J, et al. 2020. Cell Metabolism. 31(5):1017-1031. PubMed Haynes BA, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2168. PubMed
RRID: AB_10643590 (BioLegend Cat. No. 303119) AB_10640734 (BioLegend Cat. No. 303120)
Structure: Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution: Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset
Function: Cell adhesion, signal transduction
Ligand/Receptor: CD38
Cell Type: Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: DeLisser H, et al. 1994. Immunol. Today 15:490. Newman P, 1997. J. Clin. Invest. 99:3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
Gene ID: 5175
UniProt: View information about CD31 on UniProt.org
Clone: WM59
Regulatory Status: RUO
Workshop: V P025
Other Names: PECAM-1, EndoCAM
Isotype: Mouse IgG1, κ