Biolegend, 303419, Purified anti-human CD33 (Maxpar® Ready) Antibody, 100μg

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
100μg
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human myeloid leukaemia cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB) Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
Product Citations: Fox-Fisher I, et al. 2022. Med (N Y). 3:468. PubMed Gañán-Gómez I, et al. 2022. Nat Med. . PubMed Cheung P, et al. 2018. Cell. 173:1385. PubMed Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
RRID: AB_2562818 (BioLegend Cat. No. 303419)
Structure: Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution: Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
Function: Adhesion and lectin activity
Ligand/Receptor: Sugar chains containing sialic acid
Cell Type: B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: 1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. 2. Freeman S, et al. 1995. Blood 85:2005.
Gene ID: 945
UniProt: View information about CD33 on UniProt.org
Clone: WM53
Regulatory Status: RUO
Workshop: IV M-505
Other Names: Siglec-3, gp67, p67
Isotype: Mouse IgG1, κ
Q: Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
A: We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/
Q: Can I use Maxpar® Ready format clones for flow cytometry staining?
A: We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
Q: I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
A: We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
Q: Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
A: The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.