Biolegend, 304308, APC anti-human CD49d Antibody, 100tests

CD49d is a 150 kD α integrin chain known as α 4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α 4 β 1 , VLA-4) or β7 (α 4 β 7 ). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α 4 β 7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3 + cells.
100tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Cat, Cow, Chimpanzee, Common Marmoset, Dog, Horse, Sheep, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and in vitro T cell costimulation2,3. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304339 and 304340).
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim) Vogel TU, et al. 2002. J. Immunol. 169:4511. (Costim) Kleinewietfeld M, et al. 2009. Blood 113:827. (FC) PubMed Palacious F, et al. 2010. Blood 115:4488. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Mattapallil MJ, et al. 2011. J. Immunol. 187:1977. PubMed
Product Citations: Palacios F, et al. 2010. Blood. 115:4488. PubMed Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed Cossette C, et al. 2015. Biochem Pharmacol. 9: 247-255. PubMed Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed Rullo J, et al. 2012. J Cell Biol. 197:115. PubMed Caielli S, et al. 2021. Cell. 184(17):4464-4479.e19. PubMed Paris O, et al. 2022. Front Immunol. 13:975910. PubMed Bouafia A, et al. 2018. J Clin Invest. 129:1047. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Deng Y, et al. 2018. Sci Rep. 8:6777. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Shlamkovich T, et al. 2020. BMC Biology. 16(1):92. PubMed Port JR, et al. 2020. J Virol. 94:. PubMed Miller LA, et al. 2020. British Journal of Pharmacology. 177(2):360-371.. PubMed Shibata S et al. 2018. Cell reports. 25(6):1668-1679 . PubMed Deng Y, et al. 2020. J Virol. 94:00:00. PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed Genbacev O, et al. 2016. Hum Reprod. 31: 1300 - 1314. PubMed Ludwig LS et al. 2019. Cell Rep. 27(11):3228-3240 . PubMed
RRID: AB_314433 (BioLegend Cat. No. 304307) AB_2130041 (BioLegend Cat. No. 304308)
Structure: Integrin, type I transmembrane glycoprotein, 150 kD.
Distribution: T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.
Function: Lymphocyte migration, T cell activation, stem cell differentiation.
Ligand/Receptor: Fibronectin, VCAM-1, MAdCAM-1
Cell Type: B cells, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Elices M, Ed.1995. Springer Semin. Immunopathol. 16(4). 2. Lobb RR and Helmer ME. et al. 1994. J. Clin. Invest. 94:1722.
Gene ID: 3676
UniProt: View information about CD49d on UniProt.org
Clone: 9F10
Regulatory Status: RUO
Workshop: V S215
Other Names: VLA-4 α chain, α4 integrin, Integrin α4 chain, ITGA4
Isotype: Mouse IgG1, κ