Biolegend, 304313, PE/Cyanine7 anti-human CD49d Antibody, 25tests

CD49d is a 150 kD α integrin chain known as α 4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α 4 β 1 , VLA-4) or β7 (α 4 β 7 ). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α 4 β 7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3 + cells.
25tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Cat, Cow, Chimpanzee, Common Marmoset, Dog, Horse, Sheep, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and in vitro T cell costimulation2,3. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304339 and 304340).
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim) Vogel TU, et al. 2002. J. Immunol. 169:4511. (Costim) Kleinewietfeld M, et al. 2009. Blood 113:827. (FC) PubMed Palacious F, et al. 2010. Blood 115:4488. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Mattapallil MJ, et al. 2011. J. Immunol. 187:1977. PubMed
Product Citations: Dufour C, et al. 2023. Nat Commun. 14:1115. PubMed Shaw BI, et al. 2021. J Immunol. 206:1668. PubMed Giles JR, et al. 2022. Immunity. 55:557. PubMed Schaffner F, et al. 2013. PLoS One. 8:61071. PubMed Rosental B, et al. 2018. Nature. 564:425. PubMed Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed Jungwirth N et al. 2018. Journal of molecular neuroscience : MN. 65(1):60-73 . PubMed Wu HF, et al. 2022. Nat Commun. 13:7032. PubMed Evans C, et al. 2015. Haematologica. 100: 1508 - 1516. PubMed Saito‐Diaz K et al. 2019. Current protocols in stem cell biology. 49(1):e78 . PubMed Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed Kwon WY, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed Barber K, et al. 2019. Nat Protoc. 14:1261. PubMed Nasiri B, et al. 2022. Adv Healthc Mater. 11:e2200890. PubMed Olson KE, et al. 2021. EBioMedicine. 67:103380. PubMed Cederquist GY, et al. 2020. Cell Stem Cell. 27(1):35-49.e6. PubMed
RRID: AB_10642817 (BioLegend Cat. No. 304313) AB_10643278 (BioLegend Cat. No. 304314)
Structure: Integrin, type I transmembrane glycoprotein, 150 kD.
Distribution: T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.
Function: Lymphocyte migration, T cell activation, stem cell differentiation.
Ligand/Receptor: Fibronectin, VCAM-1, MAdCAM-1
Cell Type: B cells, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Elices M, Ed.1995. Springer Semin. Immunopathol. 16(4). 2. Lobb RR and Helmer ME. et al. 1994. J. Clin. Invest. 94:1722.
Gene ID: 3676
UniProt: View information about CD49d on UniProt.org
Clone: 9F10
Regulatory Status: RUO
Workshop: V S215
Other Names: VLA-4 α chain, α4 integrin, Integrin α4 chain, ITGA4
Isotype: Mouse IgG1, κ