Biolegend, 304412, Biotin anti-human CD51/61 Antibody, 100μg

CD51/CD61 is an integrin complex known as α V β 3 . It is expressed at high levels on osteoclasts, endothelial cells, and melanoma cells and at low levels on platelets and macrophages. CD51 is a heterodimer composed of disulfide-linked 125 kD and 24 kD proteins. CD61 is also a member of the integrin family known as gpIIIa or β 3 integrin. It is a 110 kD common β subunit of CD51/CD61 or CD41/CD61 complex. CD51/CD61, also known as the vitronectin receptor, mediates the binding of platelets to immobilized vitronectin without prior activation. Other ligands include RGD-containing proteins such as fibrinogen, fibronectin, von Willebrand factor (vWf), laminin, thrombospondin and the neural adhesion molecule L1. CD51/CD61 also mediates cell-cell adhesion via interaction with CD31. CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin and other RGD-containing proteins, including matrix components. The 23C6 antibody has been reported to be useful for blocking studies.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Chicken, Cynomolgus, Rabbit
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation5, immunohistochemical staining of acetone-fixed frozen tissue sections5, immunofluorescence microscopy5, and blocking of cell adhesion4,6. The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304414).
Application References(PubMed link indicates BioLegend citation): Knapp WB, et al. 1989. Leucocyte Typing IV Oxford University Press. New York. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Horton M, et al. 1991. Exp. Cell Res. 195:368. Takahashi R, et al. 1999. Blood 93:1951. (Block) Davies J, et al. 1989. J. Cell Biol. 109:1817. (IF, IHC, IP) Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709. (FC, Block) Barau A, et al. 2010. J. Ultrasound Med. 29:173. PubMed
Product Citations: Langeveld SAG, et al. 2022. Pharmaceutics. 14:. PubMed Beekers I, et al. 2020. J Control Release. 322:426. PubMed Beekers I, et al. 2020. Ultrasound Med Biol. 46:2017. PubMed
RRID: AB_528847 (BioLegend Cat. No. 304412)
Structure: Integrin, CD51/CD61 heterodimer, 24 kD
Distribution: Platelets, B subset, activated T cells, monocytes/macrophages, osteoblasts, endothelial cells, melanoma
Function: Adhesion, bone metabolism and apoptosis
Ligand/Receptor: Vitronectin, von Willebrand factor, fibrinogen, thrombospondin, osteopontin, denatured collagen
Cell Type: B cells, Endothelial cells, Macrophages, Monocytes, Osteoclasts, Platelets, T cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Davies J, et al. 1989. J. Cell Biol. 109:1817. 2. Nesbitt S, et al. 1993. J. Biol. Chem. 268:16737.
Gene ID: 36853690
UniProt: View information about CD51 CD61 on UniProt.org
Clone: 23C6
Regulatory Status: RUO
Workshop: V S246
Other Names: Vitronectin receptor, Integrin αV/β3, ITGAV, ITGB3
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.