Biolegend, 304608, PE/Cyanine5 anti-human CD56 (NCAM) Antibody, 100tests

CD56 is a single transmembrane glycoprotein also known as N-CAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
100tests
Verified Reactivity: Human
Reported Reactivity: Cow, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KG-1 human acute myelogenous leukemia cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, and Western blotting (non-reducing).
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Product Citations: Buffone A, et al. 2018. J Cell Sci. 131:. PubMed Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed Stremming J, et al. 2022. Front Physiol. 13:954948. PubMed Yang B, et al. 2015. Cell Immunol. Available online 12 August 2015. PubMed Jung J, et al. 2019. Cell Rep. 26:1906. PubMed Chang EI, et al. 2021. Front Endocrinol (Lausanne). 12:785242. PubMed Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
RRID: AB_314450 (BioLegend Cat. No. 304608)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein, 175-185 kD
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparin sulfate
Cell Type: Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: MEM-188
Regulatory Status: RUO
Workshop: VI NK26
Other Names: NCAM, Leu-19, NKH1
Isotype: Mouse IgG2a, κ