Biolegend, 304806, PE anti-human CD62L Antibody, 100tests

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Concentrated supernatant from PMA-activated human peripheral blood leukocytes
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 304853-304858).
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block) Jutila M, et al. 2002. J. Immunol. 169:1768. (WB) Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB) Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Product Citations: Liu B, et al. 2015. J Virol. 89: 11834 - 11844. PubMed Guo A, et al. 2022. Nature. 607:135. PubMed Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Chen J, et al. 2023. Cell Res. 33:341. PubMed Hagel J, et al. 2021. J Immunol. 206:3073. PubMed Li L, et al. 2022. FEBS J. 289:2877. PubMed Wohlgemuth L, et al. 2022. Cells. 11: . PubMed Cao B, et al. 2022. Nat Commun. 13:6203. PubMed Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed Chen M, et al. 2021. Cancers (Basel). 13:. PubMed Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed Jones N, et al. 2021. Nat Commun. 12:1209. PubMed Sung BY, et al. 2022. J Clin Invest. 132:. PubMed Hug S, et al. 2021. Front Immunol. 12:642867. PubMed Gatla H, et al. 2022. Front Med Technol. 4:850565. PubMed Wu D, et al. 2020. Biomark Res. 8:3. PubMed Carnevale J, et al. 2022. Nature. 609:174. PubMed Velázquez–Avila M, et al. 2019. Leukemia. 33:1337. PubMed Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed He Z, et al. 2016. Exp Hematol. 44:161-165. PubMed Burgess M, et al. 2013. Clin Cancer Res. 19:5675. PubMed Ye Z, et al. 2021. Nat Commun. 12:907. PubMed Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed Uniken Venema WT, et al. 2019. Gastroenterology. 156:812. PubMed Seery V, et al. 2021. EBioMedicine. 67:103357. PubMed Bonte S, et al. 2021. Oncoimmunology. 10:1954800. PubMed Fan Z, et al. 2020. STAR Protoc. 1:100012. PubMed Fang F, et al. 2021. Cell Rep. 37:109981. PubMed Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed Wei J, et al. 2019. J Immunother Cancer. 7:209. PubMed Messerer DAC, et al. 2020. Front Immunol. 11:571992. PubMed Tokatlian T, et al. 2022. J Immunother Cancer. 10:. PubMed Wu B, et al. 2022. Nat Commun. 13:2155. PubMed Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed Johnson AJ, et al. 2021. Cancer Immunol Res. 9:1047. PubMed Anderson NR, et al. 2019. Cell Adh Migr. 13:163. PubMed
RRID: AB_314465 (BioLegend Cat. No. 304805) AB_2564163 (BioLegend Cat. No. 304840) AB_314466 (BioLegend Cat. No. 304806)
Structure: Selectin, single chain glycoprotein, 74-95 kD
Distribution: Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
Function: Leukocyte homing, leukocyte tethering, rolling
Ligand/Receptor: CD34, GlyCAM, MAdCAM-1
Cell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. Kishimoto T, et al. 1991. Blood 78:805.
Gene ID: 6402
UniProt: View information about CD62L on UniProt.org
Clone: DREG-56
Regulatory Status: RUO
Workshop: V S056
Other Names: L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.